CaN/NFAT信号通路相关基因在鸡骨骼肌中的表达规律及关联分析

doi:10.11843/j.issn.0366-6964.2017.10.005

摘要/Abstract

摘要：

旨在探讨CaN/NFAT信号通路相关基因在鸡生长发育早期不同表型骨骼肌中的表达规律以及各基因之间的调控联系。利用荧光定量PCR技术检测隐性白羽鸡2种表型骨骼肌（腓肠肌外侧头和趾长伸肌）中CaN/NFAT信号通路相关基因（CnAα、CnB1、NFATc3、MEF2C和PPARGC1A）在出生后不同生长阶段（0、1、3、5、7和9周龄）的相对表达，并对这些基因的表达进行相关性分析。结果表明，除CnB1基因外，其他4个基因mRNA在2种表型骨骼肌中的表达趋势均较相似；2种表型骨骼肌中5个基因的表达从7周龄到9周龄均呈上升的趋势；同周龄2种表型骨骼肌相比，除腓肠肌外侧头PPARGC1A基因各周龄表达均高于趾长伸肌外，其他4个基因表达均是在3周龄时腓肠肌外侧头高于趾长伸肌，而在7和9周龄时则低于趾长伸肌；相关分析结果表明，除CnAα与NFATc3、CnAα与PPARGC1A基因的表达在2种表型肌肉中呈负相关外，其余各基因表达之间均呈正相关。提示，鸡骨骼肌CnAα、CnB1、NFATc3、MEF2C和PPARGC1A基因表达发育模式具有年龄和表型特异性，可能参与调控鸡骨骼肌肌纤维的生长和类型转换。

Abstract:

The objective of the present study was to investigate the expression profile of CaN/NFAT-dependent pathway genes, and their correlation analysis in 2 phenotypically distinct skeletal muscles were studied in chicken during the early development. qRT-PCR was used to detect the mRNA expression of CnAα, CnB1, NFATc3, MEF2C and PPARGC1A genes in lateral gastrocnemius and extensor digitorum longus of Recessive White chicken at 0,1,3,5,7 and 9 week age, the correlation among expression of these genes was analyzed. The results showed that the expression trends of all the genes between 2 phenotypically distinct skeletal muscles were similar except CnB1, and the expression level of these genes in 2 phenotypically distinct skeletal muscles was all raised from 7 to 9 week age. Comparison among the expression of these genes in 2 phenotypically distinct skeletal muscle showed that the expression level of PPARGC1A in the lateral gastrocnemius was higher than that in the extensor digitorum longus at each detected age, and the expression level of other 4 genes in the lateral gastrocnemius was higher at 3 week age and lower at 7 and 9 week age than that in the extensor digitorum longus. Correlation analysis among the 5 genes expression levels in the 2 phenotypically distinct skeletal muscles showed that there was a negative correlation between the CnAα and 2 genes (NFATc3 and PPARGC1A), and positive correlations among the expression of other genes. Overall, the expression patterns of CnAα, CnB1, NFATc3, MEF2C and PPARGC1A genes in chicken skeletal muscle are age and phenotype specific, which may be related to the regulation of the myofiber growth and type transition.

中图分类号:

S813.2

盛中伟, 单艳菊, 束婧婷, 章明, 姬改革, 屠云洁, 范建华, 邹剑敏. CaN/NFAT信号通路相关基因在鸡骨骼肌中的表达规律及关联分析[J]. 畜牧兽医学报, 2017, 48(10): 1825-1832.

SHENG Zhong-wei, SHAN Yan-ju, SHU Jing-ting, ZHANG Ming, JI Gai-ge, TU Yun-jie, FAN Jian-hua, ZOU Jian-min. The Expression Profile of CaN/NFAT-Dependent Pathway Genes and Their Correlation Analysis in Chicken Skeletal Muscle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1825-1832.

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